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60 mL Sterile phosphate buffered saline (PBS)250 mL BeakerHot pad10 Disposable sterile transfer pipette8 Disposable sterile plate spreader(6) 5 cm. Petri dishesNutrient agarParafilm1 Pair of gloves10 mL Graduated CylinderPermanent marker*Microwave or other boiling water bath*1 tsp. Soil sample*10% Bleach solution*You must provide