1. Briefly describe the basic principles of using absorption spectroscopy to determine the…
1. Briefly describe the basic principles of using absorption spectroscopy to determine the concentration of a substance in a solution.
2. Why is a reference solution needed when determining the absorbance? Why is a blank solution usually used as the reference?
3. Each time after the wavelength is changed, transmittance “0” and “100%” need to be readjusted. Explain why. Please refer to the relationship between the light absorption of a substance and the wavelength.4. What are the functions of hydroxylamine hydrochloride and sodium acetate?