7 Eukaryotic Cell StructureBackgroundCells come in all different sizes and shapes. They are classified into two major groups based on structure. Prokaryotic cells are much smaller and do not contain any membrane-bound organelle. Eukaryotic cells are larger and contain numerous membrane-bound organelles. Prokaryotic cells will be examined in the laboratory exercise that will survey the diversity of life on earth. This laboratory exercise will focus on the structure of the two most common types of eukaryotic cells: plant and animal cells.To appreciate the cell in action, watch The Inner Life of the Cellby BioVisions at Harvard University (3 min 12 sec). This video  shows movement of organelles and substances inside your cells in real time.PurposePrepare wet mounts of plant and animal cells for microscope observation.Use the light microscope to compare and contrast the structure of plant vs. animal cells.Materials (demonstrated in video)MicroscopeMicroscope slides  and cover slipsToothpickWater (or 0.9% sodium chloride solution)Medicine dropperMethylene blue (or iodine) stainElodea plantCheek cellsOnline: Virtual microscope with plant slideProcedure for plant cellView how to prepare a wet mount of an Elodealeaf (2 min 30 sec) ORhow to prepare a wet mount https://youtu.be/UWbGZMO4o_U(2 min 30 sec)To create a wet mount of an Elodea leaf, this general procedure was followed.Put a drop of water on the center of a slide.Cut an extremely small piece of an Elodea leaf and place it on the water.Place a coverslip onto the slide.Observe the plant cell slide under the virtual microscope by following this procedure.Go to the virtual microscope.Click Launch and then ExploreClick the “?” in the microscope slide boxIn the slide catalogue, select “Plant Slides”.Inside, select “Plant Cells.” The virtual microscope loads the Plant cell slide at the center of the stage. You will see a blurry green field of view.Click “10X” (low power objective lens). Watch as the nosepiece moves until the low power objective lens clicks in. Use your mouse to adjust the Coarse Focusuntil the image is in focus. Fine tune it using the Fine Focus. Slowly slide the Light Adjust to the right to obtain optimum brightness. Click on the field of view to move the plant side. Draw the image of plant cells in the field of view for low power.Click “40X” (high power objective lens). This time, only adjust the Fine Focus & Light Adjust to obtain the clearest image with optimum light. Moving the Coarse Focus will endanger the lens & slide and make you lose your focus.Draw the image in the field of view for high power.Drawing of plant cells: label cell and observed organelles.Procedure for animal cellView Cheek Epithelial Cells (3 min 52 sec) to observe how to prepare a wet mount of human cheek cell and view images of the cheek cells under low & high power magnification.To create a wet mount of cheek cells, this general procedure was followed.Put a drop of methylene blue on a slide. Caution: methylene blue stains clothes and skin.Gently scrape the inside of your cheek with the flat side of a toothpick.Stir the end of the toothpick in the stain and then throw the toothpick away.Place a coverslip onto the slide by lowering it at a 45 degree angle to prevent formation of air bubbles.Draw the cheek cell images under low & high power magnification as seen in the video.Discussion/ConclusionStains may be used but not all organelles are seen, why?Describe the shape of the plant cell. List the organelles that were visible.Describe the shape of the human cell. What organelles of the cell were visible?Describe visible similarities and differences between plant and animal cells.What observations support the claim that plant and animal cells are eukaryotic?

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I only need the graph part, otherwise the whole work is done

2. For the cost table obtained, build the following charts and answer the corresponding question:
2.1. Graph: total fixed cost, total variable cost and total cost.
2.2. Question: why is there a straight line?
2.3. Graph: average fixed cost, average variable cost, average total cost.
2.4. Question: Why does the average variable cost curve show an increasing trend while
the other two curves show a decreasing trend?
2.5. Graph: total variable cost, total fixed cost, total cost, revenue, profit or loss.
2.6. Graph: average total cost, marginal cost, marginal income.

The done work is attached below

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If you enter the field of law enforcement, you may one day have to consider using deadly force on another person, either in self-defense or to save others. Is this something you think you could be morally and ethically capable of doing? As you participate in this discussion, you will consider the moral and ethical aspects of deadly force.

Please respond to all of the following prompts in the class discussion section of your online course: 

• In what cases might it be acceptable to use deadly force? Give two examples and defend your choices.
• What are the ethical ramifications of using deadly force?
• If you headed a police squad, would you permit deadly force to be used and under what circumstances? Why or why not?

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Discuss one (1) project where you used a problem-solving approach to address what turned out to be common-cause variation, or where you used a process improvement approach to deal with a special cause.

If you do not have a personal experience that echoes either of these situations, you may use Internet to search for a case that reflects either of these situations.

Examples:

• one’s personal investment strategy since 2008
• reducing waiting times at the local hospital or emergency room
• reducing difficulties trying to connect to a Wi-Fi Internet provider

 Answer the following questions in the space provided below:

1. Describe the experience in the project.
2. What were the solutions used to address the problem?
3. Was the case you described a special-cause or common-cause?
4. Do you feel the solution or approach used appropriate for the cause?
5. What would you do if you could do it again?
6. What conclusions can you draw from the problem-solving or process-improvement techniques?

Note: You may create and / or make all necessary assumptions needed for the completion of this assignment. In your original work, you may use aspects of existing processes from either your current or a former place of employment. However, you must remove any and all identifying information that would enable someone to discern the organization(s) that you have used.

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